Coline Pinese
Coline Pinese
Associate professor, Faculty of Pharmacy, University of Montpellier
Coline has been working at the chemistry/biology interface since her doctorate CIFRE obtained in 2014. Her objective was to design hybrid collagen/PLA based copolymer biomaterials for ligament regeneration. She then continued her work at the interface by studying bioactive surfaces functionalized by peptides (antibacterial, osteointegration, wound healing…). Then she became involved into spinal cord regeneration through an association mRNA or siRNA/ nanofibers at Nayang Technological University, Singapore. She finally joined the DBA team as an Associate Professor to develop polymer/peptide hybrid nanomaterials.
Contact:
tel: +33 4 11 75 97 10
coline.pinese@umontpellier.fr
5 recent publications:
- W.Ong, C. Pinese, SY Chew, Scaffold-mediated Sequential Drug/Gene Delivery to Promote Nerve Regeneration and Remyelination following Traumatic Nerve Injuries, Advanced Drug Delivery Reviews (2018) (IF=17.28)
- Pinese, JQ. Lin, U. Milbreta, Y. Wang, KW. Leong, SY Chew, Sustained delivery of siRNA/mesoporous silica nanoparticle complexes from nanofiber scaffolds for long-term gene silencing. Acta Biomater. 76, 164–177 (2018). (IF=6.38)
- Gangolphe, S. Déjean, A. Bethry, S. Hunger, C. Pinese, X. Garric, F. Bossard, B. Nottelet, Degradable multi(aryl-azide) star copolymer as universal photo-crosslinker for elastomeric scaffolds, Materials Today Chemistry 12, (2019) 209-221 (IF=NA)
Development of hybrid bioactive nano fibers composed of star Poly (lactic acid ) and gelatin by sol – gel crosslinking during the electrospinning process
Nanotechnology 34 (2023) 485701
Karima Belabbes, Matthieu Simon, Christopher Yusef Leon-Valdivieso, Mathilde Massonié, Audrey Bethry, Gilles Subra, Xavier Garric and Coline Pinese
ABSTRACT
The design of a biomimetic scaffold is a major challenge in tissue engineering to promote tissue reconstruction. The use of synthetic polymer nano fi bers is widely described as they provide biocompatible matrices whose topography mimics natural extracellular matrix ( ECM ) . To closely match the biochemical composition of the ECM, bioactive molecules such as gelatin are added to the nano fi bers to enhance cell adhesion and proliferation. To overcome the rapid solubilization of gelatin in biological fl uids and to allow a lasting biological effect, the covalent crosslinking of this macromolecule in the network is crucial. The sol – gel route offers the possibility of gentle crosslinking during shaping but is rarely combined with electrospinning. In this study, we present the creation of Poly ( lactic acid )/ Gelatin hybrid nano fi bers by sol – gel route during electrospinning. To enable sol – gel crosslinking, we synthesized star-shaped PLA and functionalized it with silane groups; then we functionalized gelatin with the same groups for their subsequent reaction with the polymer and thus the creation of the hybrid nanonetwork. We evaluated the impact of the presence of gelatin in Poly ( lactic acid )/ Gelatin hybrid nano fi bers at different percentages on the mechanical properties, nanonetwork crosslinking, degradation and biological properties of the hybrid nano fi bers. The addition of gelatin modulated nanonetwork crosslinking that impacted the stiffness of the nano fi bers, resulting in softer materials for the cells. Moreover, these hybrid nano fi bers also showed a signi fi cant improvement in fi broblast proliferation and present a degradation rate suitable for tissue reconstruction. Finally, the bioactive hybrid nano fi bers possess versatile properties, interesting for various potential applications in tissue reconstruction.
Keywords: silylated star PLA, silylated gelatin, hybrid 3D network, bioactive scaffolds, tissue reconstruction
Design of Hybrid Polymer Nanofiber/Collagen Patches Releasing IGF and HGF to Promote Cardiac Regeneration
Eloise Kerignard, Audrey Bethry, Chloé Falcoz, Benjamin Nottelet and Coline Pinese
ABSTRACT
Cardiovascular diseases are the leading cause of death globally. Myocardial infarction in particular leads to a high rate of mortality, and in the case of survival, to a loss of myocardial functionality due to post-infarction necrosis. This functionality can be restored by cell therapy or biomaterial implantation, and the need for a rapid regeneration has led to the development of bioactive patches, in particular through the incorporation of growth factors (GF). In this work, we designed hybrid patches composed of polymer nanofibers loaded with HGF and IGF and associated with a collagen membrane. Among the different copolymers studied, the polymers and their porogens PLA-Pluronic-PLA + PEG and PCL + Pluronic were selected to encapsulate HGF and IGF. While 89 and 92% of IGF were released in 2 days, HGF was released up to 58% and 50% in 35 days from PLA-Pluronic-PLA + PEG and PCL + Pluronic nanofibers, respectively. We also compared two ways of association for the loaded nanofibers and the collagen membrane, namely a direct deposition of the nanofibers on a moisturized collagen membrane (wet association), or entrapment between collagen layers (sandwich association). The interfacial cohesion and the degradation properties of the patches were evaluated. We also show that the sandwich association decreases the burst release of HGF while increasing the release efficiency. Finally, we show that the patches are cytocompatible and that the presence of collagen and IGF promotes the proliferation of C2C12 myoblast cells for 11 days. Taken together, these results show that these hybrid patches are of interest for cardiac muscle regeneration.
Creation of a Stable Nanofibrillar Scaffold Composed of Star-Shaped PLA Network Using Sol-Gel Process during Electrospinning
Karima Belabbes, Coline Pinese *, Christopher Yusef Leon-Valdivieso, Audrey Bethry, Xavier Garric
ABSTRACT
PLA nanofibers are of great interest in tissue engineering due to their biocompatibility and morphology; moreover, their physical properties can be tailored for long-lasting applications. One of the common and efficient methods to improve polymer properties and slow down their degradation is sol-gel covalent crosslinking. However, this method usually results in the formation of gels or films, which undervalues the advantages of nanofibers. Here, we describe a dual process sol-gel/electrospinning to improve the mechanical properties and stabilize the degradation of PLA scaffolds. For this purpose, we synthesized star-shaped PLAs and functionalized them with triethoxysilylpropyl groups (StarPLA-PTES) to covalently react during nanofibers formation. To achieve this, we evaluated the use of (1) a polymer diluent and (2) different molecular weights of StarPLA on electrospinnability, StarPLA-PTES condensation time and crosslinking efficiency. Our results show that the diluent allowed the fiber formation and reduced the condensation time, while the addition of low-molecular-weight StarPLA-PTES improved the crosslinking degree, resulting in stable matrices even after 6 months of degradation. Additionally, these materials showed biocompatibility and allowed the proliferation of fibroblasts. Overall, these results open the door to the fabrication of scaffolds with enhanced stability and prospective long-term applications
Electrospun microstructured PLA-based scaffolds featuring relevant anisotropic, mechanical and degradation characteristics for soft tissue engineering
Materials Science and Engineering: C Volume 129, October 2021, 112339
Louis Gangolphe, Christopher Y.Leon Valdivieso, Benjamin Nottelet, Stéphane Déjean, Audrey Bethry, Coline Pinese, Frédéric Bossard and Xavier Garric
Electrospun scaffolds combine suitable structural characteristics that make them strong candidates for their use in tissue engineering. These features can be tailored to optimize other physiologically relevant attributes (e.g. mechanical anisotropy and cellular affinity) while ensuring adequate degradation rates of the biomaterial. Here, we present the fabrication of microstructured scaffolds by using a combination of micropatterned electrospinning collectors (honeycomb- or square-patterned) and poly(lactic acid) (PLA)-based copolymers (linear or star-shaped). The resulting materials showed appropriate macropore size and fiber alignment that were key parameters to enhance their anisotropic properties in protraction. Moreover, their elastic modulus, which was initially similar to that of soft tissues, gradually changed in hydrolytic conditions, matching the degradation profile in a 2- to 3-month period. Finally, honeycomb-structured scaffolds exhibited enhanced cellular proliferation compared to standard electrospun mats, while cell colonization was shown to be guided by the macropore contour. Taking together, these results provide new insight into the rational design of microstructured materials that can mimic the progressive evolution of properties in soft tissue regeneration